Journal: Life Sciences

Article Title: Glycolytic inhibitor 2-deoxy- d -glucose attenuates SARS-CoV-2 multiplication in host cells and weakens the infective potential of progeny virions

doi: 10.1016/j.lfs.2022.120411

Figure Lengend Snippet: 2-DG treatment reduces SARS-CoV-2 induced cell death. A. Photomicrographs show the differential change in cellular morphology upon virus infection in the indicated treatment groups performed at 48 h post-infection in Vero E6 cells ( n = 4). B. In the similar experimental condition, fluorescence images of acridine orange (AO) and ethidium bromide (EB) (AO/EB; 100 μg/ml each; 1:1) were presented using red and green emission channel respectively in untreated, and 2-DG treated [5 mM] Vero E6 cells (suspension form) captured under 10 × 10 X magnification (additional image panels are also provided in supplementary Fig. 6). C. Photo-morphometric analysis was carried out using algorithms from CELLSEGM and Image Processing toolbox in Matlab 2020a and violin plot (derived information of B), indicating the the median of AO/EB uptake (obtained from two independent observation) in the indicated treatment groups. The non-significant (ns) and significant change in the indicated groups were calculated with a two-tailed Student's t -test. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: For this purpose, the study employed algorithms from CELLSEGM and Image Processing toolbox in Matlab 2020a (Mathworks).

Techniques: Virus, Infection, Fluorescence, Suspension, Derivative Assay, Two Tailed Test